Outgoing
| Rider | Team 1965 |
|---|---|
 Sergio Alzani | Retired |
| Umberto Antoniacci | Retired |
| Alberto Assirelli | Retired |
| Ercole Baldini | Retired |
| Giovanni Garau | Retired |
| Franco Magnani | Retired |
| Ottavio Marchesi | Retired |
| Bruno Mealli | |
| Armando Pellegrini | Retired |
| Romano Piancastelli | Retired |
| Pietro Scandelli | |